NCI-H358(ICGE-NCI-H358,IC-82490)
Catalog No.
Size
Price
IC-82490
10^6
$467.00
IC-82490
10^6
�513.70
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | lung/bronchiole; derived from metastatic site: alveolus |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | bronchioalveolar carcinoma; non-small cell lung cancer |
| Gender | male |
| Applications |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy.
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.
|
| Storage Conditions | liquid nitrogen vapor temperature |
Properties
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|---|---|
| Derivation |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy.
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| Clinical Data |
male
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| Genes Expressed |
lung surfactant associated protein A (SP-A),The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.
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| Cellular Products |
lung surfactant associated protein A (SP-A)
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| Tumorigenic | Yes |
| Effects |
Yes, the cells produce tumors in athymic nude mice
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| Comments |
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.
The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.
SP-B and SP-C RNA was not expressed.
They have a reported colony forming efficiency of 0.83% in soft agarose.
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Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
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| Culture Conditions |
Temperature: 37°C
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