Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Permits and Restrictions	View Restrictions
Organism	Homo sapiens, human
Tissue	lung
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	mucoepidermoid pulmonary carcinoma
Age	32 years
Gender	female
Ethnicity	Black
Applications	The line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	This is a human cell line with near-diploid chromosome counts. The modal chromosome number was 47, occurring in 36% of cells. The rate of cells with a higher ploidy count was 3.9%. Twelve markers were common to most cells. Among them were del(1) (q32.1), der (5)t(5;13) (p15.33;q11), i(5p), der(1)t(1;?) (p34.3;?) and der (6)t(6;7) (p25.3;q21.2). All markers were present in single copy per cell. Normal N1 and N6 were absent. There were two normal X chromosomes. No other abnormalities were detected.
Derivation	This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma. The cells were isolated in a chemically defined medium (HITES) and later adapted to growth in media supplemented with serum.
Clinical Data	32 years Black female
Genes Expressed	keratin; vimentin,The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. TANK cells show reactivity with human alloantisera specific for the HLA A1, A3, A10, A19, B12, B17, B22 and B40 cross-reactive groups.
Cellular Products	keratin; vimentin
Tumorigenic	Yes
Effects	Yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features
Virus Susceptibility	Hepatitis B virus
Comments	The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase. The cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%