NCI-H1703(ICGE-NCI-H1703,IC-82482)
Catalog No.
Size
Price
IC-82482
10^6
$467.00
IC-82482
10^6
�513.70
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | lung |
| Cell Type | squamous cell |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | lung; squamous cell; non-small cell lung cancer |
| Age | 54 years adult |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Derivation |
NCI-H1703 was derived from a stage 1 lung squamous cell carcinoma of a 54-year-old Caucasian male smoker. |
|---|---|
| Clinical Data |
54 years adult
Caucasian
male
The tissue donor was a smoker (50 pack years).
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Two to three times weekly
|
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
