SK-MES-1(ICGE-SK-MES-1,IC-83449)
Catalog No.
Size
Price
IC-83449
10^6
$467.00
IC-83449
10^6
�513.70
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | lung; Derived from Metastatic Site: pleural effusion |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | squamous cell carcinoma |
| Age | 65 years adult |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype | The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining. |
|---|---|
| Clinical Data |
65 years adult
Caucasian
male
|
| Antigen Expression |
Blood Type O; Rh+; HLA A3, Aw30, B7, B27
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for use with a 5% CO2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC® Catalog No. 30-2020 (500 mL) and ATCC® Catalog No. 30-2021 (100 mL). |
|---|---|
| Subculturing |
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
|
