Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Organism	Homo sapiens, human
Tissue	cervix
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	2 [Cells contain human papilloma virus (HPV-18)] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	adenocarcinoma
Age	31 years
Gender	female
Ethnicity	Black
Applications	This cell line is a suitable transfection host. The HeLa S3 clone has been very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition, and plaque-forming ability.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	A medium-sized metacentric marker is present in 100% of the cells. HeLa Markers: One copy of M1, one copy of M2, two copies of M3, and one copy of M4. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

Derivation	HeLa S3 is a clonal derivative of the parent HeLa line (seeATCC CCL-2). S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura.
Clinical Data	31 years Black female
HeLa Markers	Y
Genes Expressed	The cells are positive for keratin by immunoperoxidase staining. keratin.
Cellular Products	keratin
Virus Susceptibility	Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) Encephalomyocarditis virus Human adenovirus 5
Virus Resistance	Human poliovirus 1 Human poliovirus 2 Human poliovirus 3
Comments	This line can be adapted to grow in suspension. The cells are positive for keratin by immunoperoxidase staining. A culture at approximately passage 400 was submitted to the American Type Culture Collection in February, 1972. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. ATCC confirmed this cell line is positive for the presence of Papillomavirus viral DNA sequences via PCR.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Flasks do not become 100% confluent. Cells are rounded and have a tendency to float in the medium. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C