SJSA-1(ICGE-SJSA-1,IC-83208)
Catalog No.
Size
Price
IC-83208
10^6
$498.00
IC-83208
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue |
bone
|
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | osteosarcoma; multipotential sarcoma |
| Age | 19 years adult |
| Gender | male |
| Ethnicity | Black |
| Storage Conditions | liquid nitrogen vapor temperature |
Properties
| Karyotype | 44XY; -1, -4, -7, -9, -11, -14, -15, +del(1)(q32;q42),+5p+, +der(7)t(4;HSR;7)(4qter-4q12::HSR::7p22--7qter), +9p+ and markers; amplified gli sequences were contained in the HSR in derivative No. 7 chromosomes |
|---|---|
| Derivation |
The SJSA-1 cell line (formerly OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur.
|
| Clinical Data |
male
19 years
Black
|
| Comments |
There is amplification of the gene which encodes the p53 associated protein, MDM2.
SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Two to three times per week
|
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
|
| Culture Conditions |
Temperature: 37°C
|
