CCRF-CEM(ICGE-CCRF-CEM,IC-39670)
Catalog No.
Size
Price
IC-39670
10^6
$532.00
IC-39670
10^6
�585.20
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | peripheral blood |
| Cell Type | T lymphoblast |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | acute lymphoblastic leukemia |
| Age | 4 years juvenile |
| Gender | female |
| Ethnicity | Caucasian |
| Applications |
This cell line is a suitable transfection host.
|
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype |
modal number = 47; range = 41 to 95. Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted. |
|---|---|
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| Derivation | Human lymphoblasts from peripheral blood of a child with acute leukemia. |
| Clinical Data |
Caucasian
female
4 years juvenile
|
| Antigen Expression |
CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD5; Homo sapiens, expressed
CD7; Homo sapiens, expressed
|
| Genes Expressed |
CD3; Homo sapiens, expressed ,CD5; Homo sapiens, expressed ,CD7; Homo sapiens, expressed ,CD4; Homo sapiens, expressed
|
| Tumorigenic | Yes |
| Effects |
Yes, in syrian hamsters
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 to 3 X 105 and 1 to 2 X 106 viable cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 7.5% DMSO.
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
|
