SW579(ICGE-SW579,IC-3779)
SW579(ICGE-SW579,IC-3779)
SW579(ICGE-SW579,IC-3779)
Catalog No.
Size
Price
IC-3779
10^6
$532.00
IC-3779
10^6
€585.20
Overview
Organism Homo sapiens, human
Tissue
thyroid
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1



Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease squamous cell carcinoma
Age 59 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phas
Properties
Clinical Data
59 years
Caucasian
male
Antigen Expression
Antigen expression: blood type O; Rh+
Genes Expressed
blood type O; Rh+
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Background
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz''s L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37¡ãC to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37¡ãC.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 37¡ãC
InCellGene


Copyright @ 2003-2024 InCellGene LLC.
twitter.com
facebook.com
linkedin.com
dribbble.com