LS180(ICGE-LS180,IC-13090)
Catalog No.
Size
Price
IC-13090
10^6
$498.00
IC-13090
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | colon |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Dukes'' type B, colorectal adenocarcinoma |
| Age | 58 years |
| Gender | female |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
Properties
| Karyotype |
modal number = 45; range = 42 to 47. The stemline karyotype was 45,t(X;5) (q12;q31) with tetraploid-like cells occurring at 2.2 percent. Occasional changes in karyotypes were also noted as a result of balanced translocations producing new marker chromosomes. In conjunction with this, decreases in chromosomes involving interchromosomal exchanges were seen. The karyotype was exactly the same as that originally described by the depositor. |
|---|---|
| Clinical Data |
female
58 years
Caucasian
|
| Antigen Expression |
serologically defined colon cancer antigen 3; Homo sapiens, expressed
HLA A2, B13, B50; Blood type O
|
| Genes Expressed |
carcinoembryonic antigen (CEA), interleukin 10, interleukin 6, mucin
The production of CEA in the ATCC seed stock was 1916 ng per 10(6) cells in 10 days. |
| Cellular Products |
carcinoembryonic antigen
interleukin 10
interleukin 6
mucin
|
| Tumorigenic | Yes. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells |
| Effects |
Yes, in nude mice
|
| Comments |
This line has never been trypsinized; however, the variant LS 174T (ATCC CL-188) has been subcultured by trypsinization.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove most medium. Dislodge cells from the floor of the flask by banging flask, washing cells by repeat pipetting and scraping gently. Aspirate and dispense into new flasks. These cells have never been trypsinized.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Two to three times per week
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
|
