Caco-2(ICGE-Caco-2,IC-39664)
Caco-2(ICGE-Caco-2,IC-39664)
Caco-2(ICGE-Caco-2,IC-39664)
Caco-2(ICGE-Caco-2,IC-39664)
Catalog No.
Size
Price
IC-39664
10^6
$498.00
IC-39664
10^6
€547.80
Overview
Permits and Restrictions

View Restrictions

Organism Homo sapiens, human
Tissue Colon
Cell Type Epithelial cells
Product Format frozen
Morphology Epithelial-like
Culture Properties Adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Colorectal adenocarcinoma
Age 72 years
Gender Male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions Liquid nitrogen vapor phase
Properties
Karyotype The stemline modal chromosome number is 96, occurring at 16% with polyploidy at 3.2%. Ten common markers were detected i.e., t(1q;?), 10q-, t(11q17q) and 7 others. The t(1q17q) and M11 were found in a portion of cells. The ins(2), 10q-, and t(15q;?) were generally paired, and t(11q;17q) and t(21q;?) were mostly three-copied. Normal N9 was absent, and N21 was lost in some cells. One to 4 small acrocentric chromosomes were detected. No Y chromosome with bright distal q-band was detected by Q-observation.


Clinical Data
72 years adult
Caucasian
male
Receptor Expression

This cell line expressed heat stable enterotoxin (Sta, E. coli) and epidermal growth factor (EGF).

Genes Expressed
keratin,retinoic acid binding protein 1,retinol binding protein 2
Cellular Products
keratin
retinoic acid binding protein 1
retinol binding protein 2
Tumorigenic Yes
Effects
Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II)
Virus Susceptibility Human immunodeficiency virus 1 , Human immunodeficiency virus 1
Comments
Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref  Caco-2 cells express retinoic acid binding protein I and retinol binding protein II. Ref 
Background
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. 
Subculturing Volumes are given for a 75 cmflask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37¡ãC to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.The recommended inoculum is 1 X 104viable cells/cm2. Subculture cells when they are about 80% confluent, at a cell concentration between 8 x 104 and 1 x 105 cell/cm2.
  6. Incubate cultures at 37¡ãC.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37¡ãC
InCellGene


Copyright @ 2003-2024 InCellGene LLC.
twitter.com
facebook.com
linkedin.com
dribbble.com