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Normal Goat Serum(IC-20012)
Click£º6760     Release date£º2008-1-1    Author£ºAdministrator    Source£ºOriginal

Normal Goat Serum(IC-20012)

Description

Normal goat serum is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

The goat serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.

Serum can be used directly for blocking, or as a constituent of a blocking buffer.

Packaging

10ml,100ml in 1 bottle.

Application

Add PBS (0.01mol/lph7.2-7.4) to dilute goat serum into 10% or 5% working solution, directly drop it on tissue sections or cell smears, incubate at 37 ¡æ for 10-30 minutes, remove the serum, do not wash, drop a proper proportion of diluted primary antibody working solution, incubate at 37 ¡æ for 1-2h or 4 ¡æ overnight.

Storage 

Aliquot and freeze undiluted product at -20¡æor below. Avoid repeated freezing and thawing.

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