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Recombinant Human GDF-15/MIC-1 |
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Cat.No.: |
CYT-Q99988 |
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Product Background |
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Human Growth/Differentiation Factor 15 / Macrophage Inhibitory Cytokine 1 |
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Growth Differentiation Factor 15 (GDF- 15), also called Macrophage Inhibitory Cytokine 1 (MIC- 1), Placental Transforming Growth Factor beta, Prostate-derived Factor, and Placental Bone Morphogenetic Protein, is a divergent member of the TGF-beta superfamily. Human GDF- 15 shares 66% and 68% amino acid sequence identity with the rat and mouse proteins, respectively. GDF- 15 is highly expressed in placenta and brain, and it is expressed at lower levels in kidney, pancreas, prostate, and colon. Similar to other TGF-beta family proteins, the GDF- 15 proprotein is cleaved at a dibasic cleavage site (RxxR) to release the mature protein. The C-terminal domain of GDF- 15 contains seven characteristic conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Biologically active GDF- 15 is a disulfide-linked homodimer of the mature protein and signals through the heterodimeric receptor composed of TGF -beta RI/ALK-5 and TGF-beta RII. GDF- 15 has been shown to have various functions, including inhibition of TNF-alpha production from lipopolysaccharide- stimulated macrophages and the induction of cartilage formation. |
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Product Information |
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Source |
E. coli |
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Molecular Weight |
Predicted molecular mass of approximately 12 kDa in SDS-PAGE under reducing condition. |
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AA Sequence |
Ala197-Ile308; Accession # Q99988 |
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Biological Activity |
Measured by its binding ability in a functional ELISA. When rHuGDF- 15/MIC- 1 is used at 0.5 ¦Ìg/mL, the concentration of Recombinant Human Activin RIB/ALK-4 Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-3 ¦Ìg/mL. |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Formulation |
Lyophilized from 0.2 ¦Ìm filtered concentrated solution in 4 mM HCl. |
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Endotoxin |
Less than 0.1 EU/¦Ìg of rHuGDF- 15/MIC- 1 as determined by LAL method. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.5 mg/mL. Further dilutions should be made in appropriately buffered solutions. |
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Purity |
> 95 % by SDS-PAGE analyses. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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12 months from date of receipt, -20 to -70 ¡ãC as supplied. |
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1 month, 2 to 8 ¡ãC under sterile conditions after reconstitution. |
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3 months, -20 to -70 ¡ãC under sterile conditions after reconstitution. |
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Usage Information |
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This product is offered by BioCytoSci only for Scientific Research & Laboratory USE. NOT FOR OTHER USE. |
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Order Information |
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Cata.No. |
Quantity |
Price($) |
Price($) |
Availability |
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CYT-Q99988 |
10ug |
$185.00 |
€203.50 |
In stock |
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CYT-Q99988 |
100ug |
$980.00 |
€1,078.00 |
In stock |
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CYT-Q99988 |
500ug |
$4,200.00 |
€4,620.00 |
In stock |
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Free Delivery on orders over $200.00. |
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We Devoted Ourselves To The Development Of Biomedical Research Reagent. |
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