Cat.No.:

CYT-P13501

Product Background

Human Regulation upon Activation Normal T cell Express Sequence/CCL5

Chemokine (C-C motif) ligand 5 (CCL5) also known as RANTES is classified as a chemotactic cytokine or chemokine. It is encoded by the CCL5 gene in humans. CCL5 is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites which has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells. It also induces the proliferation and activation of certain natural-killer cells to form CHAK cells. Recombinant human CCL5 contains 68 amino acids and it shares 84 % a.a. sequence identity with murine and rat CCL5, respectively.

Product Information

Source

E. coli

Molecular Weight

Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

AA Sequence

SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 ¦Ìm filtered concentrated solution in 20mM PB, pH 7.4, 100 mM NaCl.

Endotoxin

Less than 1 EU/¦Ìg of rHuRANTES/CCL5 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Ü -20 ¡ãC. Further dilutions should be made in appropriate buffered solutions.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 ¡ãC as supplied.

1 month, 2 to 8 ¡ãC under sterile conditions after reconstitution.

3 months, -20 to -70 ¡ãC under sterile conditions after reconstitution.

Usage Information

This  product  is  offered  by  BioCytoSci only  for Scientific Research & Laboratory  USE. NOT FOR OTHER USE.

Order Information

Cata.No.

Quantity

Price($)

Price($)

Availability

CYT-P13501

5ug

$160.00

€176.00

In stock

CYT-P13501

100ug

$960.00

€1,056.00

In stock

CYT-P13501

500ug

$3,200.00

€3,520.00

In stock

Free Delivery on orders over $200.00.

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