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Recombinant Human Basic FGF ,Basic Fibroblast Growth Factor |
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Cat.No.: |
CYT-P09038 |
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Product Background |
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Human Basic Fibroblast Growth |
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Human bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems. |
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Product Information |
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Source |
Escherichia coli. |
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Molecular Weight |
Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids |
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AA Sequence |
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP |
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Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Formulation |
Lyophilized from a 0.2 ¦Ìm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM |
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Endotoxin |
Less than 1 EU/¦Ìg of rHubFGF as determined by LAL method |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Ü -20 ¡ãC. Further dilutions should be made in appropriate buffered solutions. |
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Purity |
> 96 % by SDS-PAGE and HPLC analyses. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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12 months from date of receipt, -20 to -70 ¡ãC as supplied. |
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1 month, 2 to 8 ¡ãC under sterile conditions after reconstitution. |
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3 months, -20 to -70 ¡ãC under sterile conditions after reconstitution. |
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Usage Information |
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This product is offered by BioCytoSci only for Scientific Research & Laboratory USE. NOT FOR OTHER USE. |
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Order Information |
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Cata.No. |
Quantity |
Price($) |
Price($) |
Availability |
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CYT-P09038 |
10ug |
$156.00 |
€171.60 |
In stock |
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CYT-P09038 |
100ug |
$980.00 |
€1,078.00 |
In stock |
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CYT-P09038 |
500ug |
$4,200.00 |
€4,620.00 |
In stock |
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Free Delivery on orders over $200.00. |
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We Devoted Ourselves To The Development Of Biomedical Research Reagent. |
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