DMEM£¬High Glucose(IC-2201-S)  

Description

DMEM (Dulbecco¡¯s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Contents

This DMEM is modified as follows:

+ High Glucose(4.5g/L),

+4.0mM L-glutamine

+Phenol Red

-Sodium Pyruvate

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle¡¯s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5¨C10% CO2 environment to maintain physiological pH.

Product use

For human ex vivo tissue and cell culture processing applications.

CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using BioCytoSci® DMEM in a manufacturing process, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).

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