Insulin-Transferrin-Selenium Supplement (100X)

ITS-G,£¨IC-2125£©

(Apply For Cell Culture)

BACKGOUND 

Insulin-Transferrin-Selenium (ITS) supplementation of many conventional synthetic nutrient media permits substantial reduction in the Fetal Bovine Serum (FBS) requirement for routine maintenance and low density attachment of many adherent cell types. ITS Supplement contains Insulin, Transferrin, and Sodium Selenite, prepared in Earle¡¯s Balanced Salt Solution (EBSS) without Phenol Red. In addition, ITS-A and ITS-X contain Sodium Pyruvate and Ethanolamine, respectively. ITS and ITS-A are designed to supplement RPMI-1640 and Earle''''s Minimal Essential Medium while ITS-X is intended for use as a supplement for F-12 Nutrient Mixture, Dulbecco¡¯s Modified Eagle Medium/F-12, DMEM, and Earle''''s Minimal Essential Medium. All ITS supplements will enhance the growth of various adherent cell types at FBS concentrations less than 4%.

Formulation

Usage

1.Each 10 mL vial of Insulin-Transferrin-Selenium 100X Supplement is sufficient for up to one liter of medium.

2.In general, it is necessary to add 2¨C4% FBS to achieve optimal growth, although some adherent cultures may require less serum supplementation following initial adaptation.

3.Store Insulin-Transferrin-Selenium supplemented medium in the dark at 2¡ãC to 8¡ãC.

Important information

Insulin, transferrin, and selenium are components shown to promote optimal performance of serum-free media and reduce the need for serum supplementation of conventional media used in cell culture.

1.Insulin has pleiotropic anabolic effects on mammalian cells, promoting glucose and amino acid uptake, lipogenesis,

monovalent cation and phosphate transport, protein, and nucleic acid synthesis.

2.Transferrin serves as a carrier for iron and may also help to reduce toxic levels of oxygen radicals and peroxide.

3.Selenite is a co-factor for glutathione peroxidase and other proteins and is used as an anti-oxidant in media.

4.Pyruvate is an important precursor to amino acid, fatty acid, and cholesterol biosynthetic pathways. Pyruvate can be utilized in the Krebs'''' cycle, and in gluconeogenesis.

5.Ethanolamine can serve as a precursor for the biosynthesis of phosphoglycerides, which are essential to the structure of the plasma membrane and cellular organelles.

Size
10ml,10mlx5.

STORAGE

Store at 2-8¡æ For 18 Months.

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