Anti-LCN2 Antibody (Rabbit Polyclonal)
Catalog Number: 203934
Lot Number: Refer to vial label
Storage: -20¡ãC
PRODUCT DESCRIPTION
Target Protein: LCN2 (Lipocalin-2 / NGAL)
Gene ID: 3934
Host Species: Rabbit
Clonality: Polyclonal
Reactivity: Human (H), Mouse (M)
Applications: WB, IHC, IF
Immunogen: Recombinant peptide corresponding to LCN2 (Gene ID: 3934)
Purification Method: Antigen affinity chromatography
Formulation: Liquid in PBS (pH 7.4) with 0.02% sodium azide and 50% glycerol
Isotype: IgG
RECOMMENDED DILUTIONS & PROTOCOLS
Western Blot (WB)
Dilution: 1:500 - 1:2000
Positive control: Mouse kidney or human HEK-293 cell lysate
Expected band size: ~22-25 kDa
Immunohistochemistry (IHC)
Dilution: 1:100 - 1:500
Antigen retrieval: Sodium citrate buffer (pH 6.0) with heat
Immunofluorescence (IF)
Dilution: 1:100 - 1:500
Fixation: 4% PFA
Permeabilization: 0.1% Triton X-100
STORAGE INSTRUCTIONS
Long-term storage: -20¡ãC (up to 12 months)
Short-term storage: 4¡ãC (1-2 weeks)
Avoid: Repeated freeze-thaw cycles (aliquot after first thaw)
VALIDATION & EXPECTED RESULTS
Positive control tissues: Human and mouse kidney, lung, and HEK-293 cells
Subcellular localization: Cytoplasm, secreted
WB (Mouse kidney): Single band at ~22-25 kDa
IHC (Mouse kidney): Positive staining in renal tubules
IF (HEK-293): Cytoplasmic signal
GENERAL NOTES
1. Perform pilot experiment to determine optimal dilution for your sample.
2. Include no-primary-antibody control as negative control for IHC.
3. For research use only. Not for diagnostic or therapeutic use in humans.
ORDERING INFORMATION
Catalog Number: 203934
Product Name: Anti-LCN2 Antibody
Size: 50 uL / 100 uL
Price:
50 uL: CNY ¥1600.00 / USD $160.00 / EUR €192.00 / JPY ¥28800.00
100 uL: CNY ¥2500.00 / USD $250.00 / EUR €300.00 / JPY ¥45000.00
CONTACT INFORMATION
For technical support, please provide catalog number 203934.
