Catalog Number: 206277

Lot Number: Refer to vial label

Storage: -20¡ãC

PRODUCT DESCRIPTION

Target Protein: S100A6 (Calcyclin)

Gene ID: 6277

Host Species: Rabbit

Clonality: Polyclonal

Reactivity: Human (H), Mouse (M)

Applications: WB, IHC, IF

Immunogen: Recombinant peptide corresponding to S100A6 (Gene ID: 6277)

Purification Method: Antigen affinity chromatography

Formulation: Liquid in PBS (pH 7.4) with 0.02% sodium azide and 50% glycerol

Isotype: IgG

RECOMMENDED DILUTIONS & PROTOCOLS

Western Blot (WB)

Dilution: 1:500 - 1:2000

Positive control: Mouse brain or human HeLa cell lysate

Expected band size: ~10-12 kDa

Immunohistochemistry (IHC)

Dilution: 1:100 - 1:500

Antigen retrieval: Sodium citrate buffer (pH 6.0) with heat

Immunofluorescence (IF)

Dilution: 1:100 - 1:500

Fixation: 4% PFA

Permeabilization: 0.1% Triton X-100

STORAGE INSTRUCTIONS

Long-term storage: -20¡ãC (up to 12 months)

Short-term storage: 4¡ãC (1-2 weeks)

Avoid: Repeated freeze-thaw cycles (aliquot after first thaw)

VALIDATION & EXPECTED RESULTS

Positive control tissues: Human and mouse brain, heart, and HeLa cells

Subcellular localization: Cytoplasm, nucleus, membrane

WB (Mouse brain): Single band at ~10-12 kDa

IHC (Mouse brain): Positive staining in neurons

IF (HeLa cells): Cytoplasmic and nuclear signal

GENERAL NOTES

1. Perform pilot experiment to determine optimal dilution for your sample.

2. Include no-primary-antibody control as negative control for IHC.

3. For research use only. Not for diagnostic or therapeutic use in humans.

ORDERING INFORMATION

Catalog Number: 206277

Product Name: Anti-S100A6 Antibody

Size: 50 uL / 100 uL

Price:

50 uL: CNY ¥1600.00 / USD $160.00 / EUR €192.00 / JPY ¥28800.00

100 uL: CNY ¥2500.00 / USD $250.00 / EUR €300.00 / JPY ¥45000.00

CONTACT INFORMATION

For technical support, please provide catalog number 206277.