Recombinant Mouse Endothelin-2 (Edn2), REM-P22389
Product Introduction
Endothelin-2 (Edn2), also known as vasoactive intestinal contractor (VIC), is a 21-amino acid peptide of the endothelin family that functions as a potent vasoconstrictor and signaling molecule. Endothelins are produced primarily by endothelial cells and act through two G protein-coupled receptors, ETA and ETB, to regulate vascular tone, cell proliferation, and hormone secretion. Endothelin-2 is expressed in various tissues including the intestine, ovary, lung, and placenta, where it plays roles in smooth muscle contraction, follicular rupture during ovulation, and pulmonary function. Unlike Endothelin-1, which is constitutively expressed, Endothelin-2 exhibits more restricted and regulated expression patterns, particularly during epithelial-mesenchymal interactions and inflammatory responses. This recombinant mouse Endothelin-2 protein comprises the mature active peptide region (amino acids 44-66) with an N-terminal human Fc1 tag, expressed in a mammalian cell system to ensure proper folding and disulfide bond formation. The Fc tag facilitates detection and purification while extending the circulating half-life for in vivo studies.
Product Features
1. Mammalian Cell Expression: Produced in mammalian cells, ensuring proper post-translational modifications including disulfide bond formation critical for endothelin biological activity.
2. Mature Active Peptide Region: Encompasses the mature Endothelin-2 sequence (amino acids 44-66) containing the biologically active 21-amino acid peptide.
3. N-Terminal hFc1 Tag: Human Fc1 tag facilitates detection by anti-human Fc antibodies and enables purification by Protein A/G affinity chromatography.
4. High Purity: ≥95% as determined by SDS-PAGE and SEC-HPLC, ensuring reproducibility in experimental applications.
5. Flexible Delivery Format: Available as liquid or lyophilized powder to accommodate different experimental requirements.
Specifications
Size: Available in 20 µg and 100 µg
Species: Mus musculus (Mouse)
Gene Name: Edn2
UniProt Accession: P22389
Protein Length: Partial (mature peptide region)
Expression Region: Amino acids 44-66
Amino Acid Sequence: RRCSCNSWLDKECVYFCHLDIIW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc. If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Tag: N-terminal hFc1-tagged
Source: Mammalian cell
Molecular Weight: 29.0 kDa
Purity: ≥95% as determined by SDS-PAGE; ≥95% as determined by SEC-HPLC
Biological Activity: Not tested
Aliases: Preproendothelin-2; Vasoactive intestinal contractor
Product Form: Liquid or lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer:
- Liquid form: Tris/PBS-based buffer, 5%-50% glycerol
- Lyophilized powder: Tris/PBS-based buffer, 6% Trehalose, pH 8.0 (before lyophilization)
Storage and Stability
Storage Conditions: Store at -20°C or -80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles. Working aliquots may be stored at 4°C for up to one week.
Shelf Life:
- Liquid form: 6 months at -20°C/-80°C
- Lyophilized form: 12 months at -20°C/-80°C
Note: The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself.
Reconstitution (for Lyophilized Form): Briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration, default recommendation is 50%) and aliquot for long-term storage at -20°C/-80°C.
Lead Time: 3-7 business days
Protocol (For Reference Only)
Important: This recombinant protein carries an N-terminal hFc1 tag. Verify tag compatibility with downstream applications before use. For cell-based assays, endotoxin levels should be considered; please request endotoxin data if required for your application.
1. Reconstitution and Storage: If supplied as lyophilized powder, briefly centrifuge the vial to collect contents. Reconstitute in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Add glycerol to a final concentration of 5-50% (50% recommended) for cryoprotection. Aliquot into single-use volumes and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
2. Working Solution Preparation: Thaw an aliquot on ice and dilute to the desired working concentration in appropriate buffer or cell culture medium. For cell-based receptor binding or signaling assays, typical concentrations range from 1-100 nM depending on receptor expression levels and assay sensitivity.
3. Endothelin Receptor Binding Assays: Incubate cells expressing ETA or ETB receptors with recombinant Endothelin-2 at concentrations ranging from 0.1-100 nM. Assess receptor binding by competition with radiolabeled or fluorescent endothelin ligands, or evaluate downstream signaling by measuring intracellular calcium flux, IP3 accumulation, or ERK phosphorylation.
4. Vasoconstriction and Smooth Muscle Assays: For ex vivo vascular or intestinal smooth muscle preparations, apply recombinant Endothelin-2 at concentrations of 1-100 nM in organ bath experiments. Monitor isometric tension changes and compare responses to Endothelin-1 or Endothelin-3 controls.
5. Fc Tag Detection: The N-terminal hFc1 tag can be detected using anti-human IgG (Fc-specific) antibodies in Western blot, ELISA, or immunohistochemistry applications. For applications where the Fc tag may interfere, consider tag removal using appropriate proteases.
Precautions
1. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
2. The biological activity of this recombinant protein has not been tested by the manufacturer. End-users should validate biological activity in their specific assay systems.
3. If the exact amino acid sequence is critical to your application, please explicitly request the full and complete sequence before ordering.
4. The product may be shipped as either liquid or lyophilized powder based on stock availability. If a specific format is required, specify this at the time of order.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the biological role of Endothelin-2?
A1: Endothelin-2 is a potent vasoconstrictor peptide of the endothelin family. It acts through ETA and ETB receptors to regulate vascular tone, smooth muscle contraction, and hormone secretion. Unlike Endothelin-1, it exhibits more restricted and regulated expression, with specific roles in ovulation, intestinal contraction, and pulmonary function.
Q2: Has the biological activity of this recombinant protein been tested?
A2: No. The biological activity of this recombinant protein has not been tested by the manufacturer. End-users should validate activity in their specific assay systems. The N-terminal hFc1 tag may also influence receptor binding or bioactivity.
Q3: What format will I receive the product in?
A3: The product may be shipped as either liquid or lyophilized powder based on stock availability. If you require a specific format, please specify this at the time of order, and we will prepare according to your demand.
Q4: How should I store this product after reconstitution?
A4: After reconstitution, add glycerol to a final concentration of 5-50% (50% recommended) for cryoprotection. Aliquot into single-use volumes and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Working aliquots may be stored at 4°C for up to one week.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of conditions using a small-scale pilot experiment is strongly recommended before processing valuable samples.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: REM-P22389
Product Name: Recombinant Mouse Endothelin-2 (Edn2)
Size: 20 µg / 100 µg
Price:
20 µg: CNY ¥1500.00 / USD $150.00 / EUR �180.00 / JPY ¥27000.00
100 µg: CNY ¥3075.00 / USD $307.50 / EUR �369.00 / JPY ¥55350.00
2023 Version
