Product Introduction
Inosine is a purine nucleoside composed of hypoxanthine and ribose, formed by the deamination of adenosine. It serves as an important intermediate in purine metabolism and is a precursor for the synthesis of guanine nucleotides via the purine salvage pathway. Inosine has been shown to exert immunomodulatory, neuroprotective, and cardioprotective effects. It is used as a nutritional supplement and has been investigated for its potential benefits in neurological disorders, cardiovascular conditions, and athletic performance. Inosine is also widely used in laboratory research as a substrate for purine nucleoside phosphorylase and other enzymes, and as a component of cell culture media. Additionally, inosine can be incorporated into RNA and acts as a wobble base in tRNA, contributing to codon-anticodon flexibility.

Product Features
1. Purine Nucleoside: Important intermediate in purine metabolism and guanine nucleotide synthesis.
2. Immunomodulatory Effects: Modulates immune responses and cytokine production.
3. Neuroprotective and Cardioprotective: Investigated for protective effects in neurological and cardiovascular conditions.
4. Broad Research Utility: Used as an enzyme substrate, cell culture supplement, and RNA component.
5. High Purity: Supplied at 98% purity for reproducible research applications.

Specifications
Size: 1 g / 5 g / 10 g
CAS Number: 58-63-9
Purity: 98%
Molecular Weight: 268.23
Molecular Formula: C10H12N4O5
Solubility: Water: ¡Ý10 mg/mL; DMSO: slightly soluble; Ethanol: slightly soluble
Appearance: White to off-white crystalline powder

Storage and Stability
Storage Conditions: Store at room temperature, sealed in dry conditions, and keep in a dark place. Protect from light and moisture.
Shelf Life: The product is stable for 36 months from the date of manufacture when stored as directed.

Protocol (For Reference Only)
Important: Protect from light and moisture. Prepare solutions fresh or store at 4¡ãC for short-term use.
1. Stock Solution Preparation: Dissolve inosine in sterile water or appropriate buffer to prepare a stock solution (e.g., 10-50 mM). Filter sterilize for cell culture applications. Store at 4¡ãC for short-term use or aliquot and store at -20¡ãC.
2. Cell Culture Supplementation: Supplement cell culture medium with inosine at final concentrations of 0.1-1 mM as needed for specific cell types.
3. Enzyme Substrate Assays: Use inosine as a substrate for purine nucleoside phosphorylase or other purine metabolism enzymes. Prepare assay mixtures according to the specific enzymatic assay protocol.
4. Neuroprotection and Immunomodulation Studies: Treat cells or tissues with inosine at 10-100 µM and assess relevant endpoints, including cytokine production, cell viability, and oxidative stress markers.
5. In Vivo Studies: Inosine has been administered in rodent models via oral or intraperitoneal routes at doses ranging from 10-100 mg/kg. Optimize dosing and route based on specific experimental requirements.

Precautions
1. Protect from light and moisture during storage.
2. Prepare solutions fresh or store at 4¡ãC for short-term use to avoid degradation.
3. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)
Q1: What is the biological role of inosine?
A1: Inosine is a purine nucleoside intermediate in purine metabolism and a precursor for guanine nucleotide synthesis. It also exhibits immunomodulatory, neuroprotective, and cardioprotective effects.
Q2: Can inosine be used in cell culture?
A2: Yes. Inosine can be supplemented in cell culture medium at 0.1-1 mM, depending on the specific cell type and experimental requirements.
Q3: How should I store inosine?
A3: Store at room temperature sealed in dry conditions protected from light and moisture.

Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of working concentrations is recommended for specific experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information
Catalog Number: IC-02157562
Product Name: Inosine
Size: 1 g / 5 g / 10 g
Suggested Retail Price:
1 g: CNY ¥550.00 / USD $55.00 / EUR €66.00 / JPY ¥9900.00
5 g: CNY ¥750.00 / USD $75.00 / EUR €90.00 / JPY ¥13500.00
10 g: CNY ¥1235.00 / USD $123.50 / EUR €148.20 / JPY ¥22230.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.