Recombinant Human Interleukin-18, CYT-Q14116
Product Introduction
Interleukin-18 (IL-18) is a secreted protein belonging to the IL-1 family. IL-18 is a proinflammatory cytokine produced by macrophages and other cells. This cytokine can induce IFN-γ production by T cells. The combination of IL-18 and IL-12 has been shown to inhibit IL-4-dependent IgE and IgG1 production and enhance IgG2a production by B cells. IL-18 binding protein (IL18BP) can specifically interact with IL-18 and negatively regulate its biological activity. After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine, interferon-γ (IFN-γ) or type II interferon, which plays an important role in activating macrophages and other cells.
Product Features
1. High Purity: Greater than 95% as determined by SEC-HPLC (QC verified).
2. High Biological Activity: Measured by its ability to enhance IFN-γ secretion in NK-92 human natural killer lymphoma cells, with an ED50 of 0.5-2.5 ng/mL.
3. E. coli Expression: Produced in an E. coli expression system with the target gene encoding Tyr37-Asp193.
4. Low Endotoxin: <0.01 EU/µg as determined by LAL test.
5. Lyophilized Powder: Supplied as a lyophilized powder for convenient storage and handling.
Specifications
Size: 10 µg / 50 µg
Protein Accession: Q14116
Alternative Names: Interleukin-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; GIF; IL-18; IL-1g; IL1F4; MGC12320
Molecular Weight: 18.2 kDa
Apparent Molecular Weight: 17 kDa, reducing conditions
Expression Region: Tyr37-Asp193
Formulation: Lyophilized from a 0.2 µm filtered solution of 20 mM PB, 150 mM NaCl, 5% Sucrose, 0.1% Tween 80, pH 7.4
Endotoxin: <0.01 EU/µg (LAL test)
Purity: Greater than 95% by SEC-HPLC
Biological Activity: ED50 of 0.5-2.5 ng/mL, measured by its ability to enhance IFN-γ secretion in NK-92 human natural killer lymphoma cells
Storage and Stability
Shipping Conditions: Shipped at ambient temperature. Upon receipt, store immediately at the temperature listed below.
Storage Conditions:
- Lyophilized protein: Store at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution: Store at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples: Stable at ≤ -20°C for 3 months.
Protocol (For Reference Only)
Important: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/mL.
1. Reconstitution: Dissolve the lyophilized protein in distilled water. Allow to dissolve completely with gentle mixing; do not vortex.
2. Aliquoting: Aliquot the reconstituted solution into single-use volumes to minimize freeze-thaw cycles.
3. Storage: Store aliquots at ≤ -20°C for up to 3 months. Reconstituted solution may be stored at 2-8°C for 2-7 days.
4. Activity Assay: Biological activity is measured by its ability to enhance IFN-γ secretion in NK-92 human natural killer lymphoma cells, with an ED50 of 0.5-2.5 ng/mL.
5. Working Concentration: Optimal working concentrations should be determined empirically for each cell type and experimental system.
Precautions
1. Always centrifuge tubes before opening.
2. Do not mix by vortex or pipetting; gentle inversion or swirling is recommended.
3. It is not recommended to reconstitute to a concentration less than 100 µg/mL to avoid protein loss.
4. Aliquot reconstituted solution to minimize freeze-thaw cycles.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: How should I reconstitute this protein?
A1: Dissolve the lyophilized protein in distilled water to a concentration of at least 100 µg/mL. Do not vortex or pipette vigorously.
Q2: What is the biological activity of this protein?
A2: The ED50 is 0.5-2.5 ng/mL as measured by its ability to enhance IFN-γ secretion in NK-92 human natural killer lymphoma cells.
Q3: How should I store the reconstituted protein?
A3: Reconstituted solution can be stored at 2-8°C for 2-7 days. For longer storage, aliquot and store at ≤ -20°C for up to 3 months. Avoid repeated freeze-thaw cycles.
Q4: What is the endotoxin level?
A4: <0.01 EU/µg as determined by LAL test.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of working concentrations is recommended for specific cell types and experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CYT-Q14116
Product Name: Recombinant Human Interleukin-18
Size: 10 µg / 50 µg
Suggested Retail Price:
10 µg: CNY ¥1600.00 / USD $160.00 / EUR �192.00 / JPY ¥28800.00
50 µg: CNY ¥5600.00 / USD $560.00 / EUR �672.00 / JPY ¥100800.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
