Recombinant Human IL-12, CYT-P29459
Product Introduction
IL-12 is a heterodimeric pleiotropic cytokine composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor that can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4-mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.
Product Features
1. High Purity: Greater than 95% as determined by SEC-HPLC (QC verified).
2. High Biological Activity: Measured by its ability to enhance IFN-γ secretion in NK-92 human natural killer lymphoma cells, with an ED50 of 0.2-0.8 ng/mL.
3. Mammalian Expression System: Produced in a mammalian expression system for proper folding and post-translational processing.
4. Low Endotoxin: <0.01 EU/µg as determined by LAL test.
5. Lyophilized Powder: Supplied as a lyophilized powder for convenient storage and handling.
Specifications
Size: 10 µg / 50 µg
Protein Accession: P29459
Alternative Names: Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; IL12A; NKSF1
Molecular Weight: 22.5 kDa and 34.7 kDa
Apparent Molecular Weight: 25-38 kDa and 40-50 kDa, reducing conditions
Expression Region: Arg23-Ser219 and Ile23-Ser328
Formulation: Lyophilized from a 0.2 µm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4
Endotoxin: <0.01 EU/µg (LAL test)
Purity: Greater than 95% by SEC-HPLC
Biological Activity: ED50 of 0.2-0.8 ng/mL, measured by its ability to enhance IFN-γ secretion in NK-92 human natural killer lymphoma cells
Storage and Stability
Shipping Conditions: Shipped at ambient temperature. Upon receipt, store immediately at the temperature listed below.
Storage Conditions:
- Lyophilized protein: Store at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution: Store at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples: Stable at ≤ -20°C for 3 months.
Protocol (For Reference Only)
Important: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/mL.
1. Reconstitution: Dissolve the lyophilized protein in distilled water. Allow to dissolve completely with gentle mixing; do not vortex.
2. Aliquoting: Aliquot the reconstituted solution into single-use volumes to minimize freeze-thaw cycles.
3. Storage: Store aliquots at ≤ -20°C for up to 3 months. Reconstituted solution may be stored at 2-8°C for 2-7 days.
4. Activity Assay: Biological activity is measured by its ability to enhance IFN-γ secretion in NK-92 human natural killer lymphoma cells, with an ED50 of 0.2-0.8 ng/mL.
5. Working Concentration: Optimal working concentrations should be determined empirically for each cell type and experimental system.
Precautions
1. Always centrifuge tubes before opening.
2. Do not mix by vortex or pipetting; gentle inversion or swirling is recommended.
3. It is not recommended to reconstitute to a concentration less than 100 µg/mL to avoid protein loss.
4. Aliquot reconstituted solution to minimize freeze-thaw cycles.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: How should I reconstitute this protein?
A1: Dissolve the lyophilized protein in distilled water to a concentration of at least 100 µg/mL. Do not vortex or pipette vigorously.
Q2: What is the biological activity of this protein?
A2: The ED50 is 0.2-0.8 ng/mL as measured by its ability to enhance IFN-γ secretion in NK-92 human natural killer lymphoma cells.
Q3: How should I store the reconstituted protein?
A3: Reconstituted solution can be stored at 2-8°C for 2-7 days. For longer storage, aliquot and store at ≤ -20°C for up to 3 months. Avoid repeated freeze-thaw cycles.
Q4: What is the subunit composition of this protein?
A4: IL-12 is a heterodimeric cytokine composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of working concentrations is recommended for specific cell types and experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CYT-P29459
Product Name: Recombinant Human IL-12
Size: 10 µg / 50 µg
Suggested Retail Price:
10 µg: CNY ¥1600.00 / USD $160.00 / EUR �192.00 / JPY ¥28800.00
50 µg: CNY ¥5600.00 / USD $560.00 / EUR �672.00 / JPY ¥100800.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
