RAW 264.7 Cell Culture Medium, CM-RAW264
Product Introduction
This product is a specialized complete medium carefully optimized and long-term tested to maintain the optimal growth state of RAW 264.7 cells. The medium already contains all components required for the growth of RAW 264.7 cells and requires no additional supplementation before use. It can be used directly for the culture of RAW 264.7 cells.
Product Components
DMEM Basal Medium: 445 mL
Premium Fetal Bovine Serum: 50 mL
Penicillin/Streptomycin (P/S): 5 mL
Product Features
1. Ready-to-Use Complete Medium: Contains all necessary components; no additional supplementation required.
2. Optimized Formulation: Specifically designed to maintain optimal growth of RAW 264.7 cells.
3. Quality Controlled: Each batch tested for sterility, pH, endotoxin, and cell growth performance.
Specifications
Size: 100 mL / 500 mL
Cell Type: RAW 264.7 cells
Form: Liquid complete medium
Storage and Stability
Shipping: Low-temperature transport in an insulated container with biological ice packs.
Storage Conditions: Store at 2-8°C protected from light for 2 months; store at -20°C protected from light for 6 months.
Shelf Life: 2 months at 2-8°C; 6 months at -20°C.
Quality Control
Clarity: Clear
pH: 7.3 ± 0.2
Endotoxin: ≤10 EU/mL
Sterility: Negative for bacteria, fungi, and mycoplasma
Cell Growth Test: Cell morphology normal; cell growth test qualified
Protocol (For Reference Only)
Important: Use aseptic technique throughout. Warm complete medium to 37°C before use.
1. Complete Medium Preparation: If components are provided separately, thaw the FBS and P/S at 4°C or room temperature, then add to the basal medium under aseptic conditions. Mix gently by swirling. Store the prepared complete medium at 2-8°C protected from light and use within 2 months.
2. Medium Pre-warming: Warm the required volume of complete medium to 37°C for 15-30 minutes before use.
3. Cell Culture: Seed cells in appropriate culture vessels. RAW 264.7 cells are semi-adherent and can be maintained in suspension or adherent culture depending on conditions. Incubate at 37°C, 5% CO₂.
4. Medium Change: Replace medium every 2-3 days or when pH indicator changes color.
5. Subculturing: When cells reach the desired density, gently detach using a cell scraper or gentle pipetting. Avoid vigorous trypsinization, which may damage RAW 264.7 cells. Passage according to standard protocols.
Precautions
1. Upon receipt, inspect the packaging for integrity. If breakage, leakage, or turbidity is observed, contact the supplier promptly. After freeze-thaw, small amounts of flocculent precipitate may appear in the medium; this does not affect normal product use.
2. Read the product manual carefully to understand usage, storage, and shelf life. Contamination due to improper operation or storage is the responsibility of the customer.
3. This product is for research use only. Some components in the medium have low toxicity to humans. If contact occurs, rinse immediately with copious amounts of water.
FAQ (Simplified)
Q1: Does this medium require any additional supplementation?
A1: No. This complete medium contains all components required for RAW 264.7 cell culture and requires no additional supplementation.
Q2: How should I passage RAW 264.7 cells?
A2: RAW 264.7 cells are semi-adherent and can be detached by gentle scraping or pipetting. Avoid vigorous trypsinization, which may damage the cells.
Q3: How should I store this medium?
A3: Store at 2-8°C protected from light for 2 months, or at -20°C protected from light for 6 months.
Q4: What should I do if flocculent precipitate appears after thawing?
A4: Small amounts of flocculent precipitate may appear after freeze-thaw and do not affect product performance. Mix gently before use.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data resulting from improper use or storage.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CM-RAW264
Product Name: RAW 264.7 Cell Culture Medium
Size: 100 mL / 500 mL
Suggested Retail Price:
100 mL: CNY ¥550.00 / USD $55.00 / EUR �66.00 / JPY ¥9900.00
500 mL: CNY ¥1880.00 / USD $188.00 / EUR �225.00 / JPY ¥35100.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
