0.1% Crystal Violet Staining Solution, IC-21001
Product Introduction
This product is a 0.1% crystal violet staining solution, widely used for the staining of cell nuclei, cell colonies, and tissue sections in biological research. Crystal violet is a basic triphenylmethane dye that binds to acidic components of cells, particularly DNA, producing a distinctive purple color. In cell biology, 0.1% crystal violet staining solution is commonly used for colony formation assays to visualize and quantify adherent cell colonies, for cell morphology observation, and for the assessment of cell migration and invasion in Transwell assays. The ready-to-use formulation provides convenient and reproducible staining for routine laboratory applications.
Product Features
1. Ready-to-Use: Supplied as a 0.1% solution requiring no dilution or preparation.
2. Standard Concentration: Optimized concentration for colony formation assays, cell morphology, and Transwell quantification.
3. Reliable Staining: Binds to DNA and acidic cellular components, producing clear and reproducible purple staining.
4. Versatile Application: Suitable for colony formation assays, cell migration and invasion assays, and general cell morphology observation.
Specifications
Size: 10 mL / 50 mL / 100 mL
Concentration: 0.1%
Appearance: Purple liquid
Application: Colony formation assay, cell morphology, Transwell quantification
Storage and Stability
Storage Conditions: Store at room temperature (15-25¡ãC), protected from light.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: Use appropriate personal protective equipment when handling crystal violet solution, as it may stain skin and clothing.
1. Colony Formation Assay: After colony formation, remove culture medium and wash cells gently with PBS. Fix cells with 4% paraformaldehyde or methanol for 10-15 minutes. Remove fixative and add sufficient 0.1% crystal violet staining solution to cover the cells. Stain for 10-20 minutes at room temperature. Remove staining solution and wash gently with PBS or distilled water until background is clear. Air dry and photograph or count colonies.
2. Transwell Migration/Invasion Assay: After the assay, remove non-migrated cells from the upper surface of the membrane with a cotton swab. Fix migrated cells with 4% paraformaldehyde or methanol for 10-15 minutes. Stain with 0.1% crystal violet staining solution for 10-20 minutes at room temperature. Wash and air dry. Quantify migrated cells by microscopy or elute the dye with 10% acetic acid and measure absorbance at 560-590 nm.
3. Cell Morphology Observation: Wash cells with PBS, fix with 4% paraformaldehyde or methanol, and stain with 0.1% crystal violet staining solution for 10-20 minutes. Wash and observe under a microscope.
Precautions
1. Crystal violet may stain skin and clothing; wear gloves and laboratory protective clothing when handling.
2. Protect from light during storage.
3. The staining solution may be reused several times; filter if particulate matter accumulates.
4. Dispose of crystal violet waste according to institutional guidelines.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the typical application of 0.1% crystal violet staining solution?
A1: It is commonly used for colony formation assays, Transwell migration and invasion assays, and cell morphology observation.
Q2: How long should I stain cells?
A2: Typically 10-20 minutes at room temperature is sufficient for most applications.
Q3: Can the staining solution be reused?
A3: Yes. The staining solution may be reused several times; filter if particulate matter accumulates.
Q4: How should I store this product?
A4: Store at room temperature protected from light.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of staining conditions is recommended for specific cell types and applications.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-21001
Product Name: 0.1% Crystal Violet Staining Solution
Size: 10 mL / 50 mL / 100 mL
Suggested Retail Price:
10 mL: CNY ¥145.00 / USD $14.50 / EUR €17.40 / JPY ¥2500.00
50 mL: CNY ¥250.00 / USD $25.00 / EUR €30.00 / JPY ¥4500.00
100 mL: CNY ¥370.00 / USD $37.00 / EUR €44.40 / JPY ¥6660.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
