Streptozotocin,18883-66-4, IC-03205403
Product Introduction
Streptozotocin (STZ) is a naturally occurring glucosamine-nitrosourea compound produced by Streptomyces achromogenes, widely used as a diabetogenic agent in animal models and as a chemotherapeutic agent in research. STZ is selectively taken up by pancreatic ¦Â-cells through the glucose transporter GLUT2, where it induces DNA alkylation, nitric oxide production, and oxidative stress, leading to ¦Â-cell necrosis and the development of insulin-dependent diabetes mellitus. This property makes STZ an essential tool for establishing experimental models of type 1 and type 2 diabetes, diabetic complications, and pancreatic islet biology. STZ is also used in cancer research due to its alkylating activity and ability to induce DNA damage. Its specificity for pancreatic ¦Â-cells, dose-dependent effects, and reproducible diabetogenic activity have established STZ as one of the most widely used chemical agents in metabolic disease research.
Product Features
1. ¦Â-Cell Specificity: Selectively taken up by pancreatic ¦Â-cells via GLUT2, enabling targeted induction of ¦Â-cell damage.
2. Well-Established Diabetogenic Agent: Widely used to establish type 1 and type 2 diabetes models in rodents.
3. Alkylating Activity: Induces DNA alkylation and oxidative stress, useful in cancer and DNA damage research.
4. Dose-Dependent Effects: Low doses induce diabetes, while high doses cause ¦Â-cell necrosis; dosing can be optimized for specific model requirements.
5. High Purity: Supplied at 98% purity with ¡Ý85% ¦Á-anomer basis for reproducible experimental results.
Specifications
Size: 50 mg / 100 mg / 250 mg / 500 mg / 1 g
CAS Number: 18883-66-4
Purity: 98% (¡Ý85% ¦Á-anomer basis)
Molecular Weight: 265.22
Molecular Formula: C8H15N3O7
Solubility: Water: ¡Ý50 mg/mL (prepare fresh, pH 4.5-5.5); DMSO: ¡Ý10 mg/mL
Storage and Stability
Storage Conditions: Store at -20¡ãC under inert atmosphere, protected from light and moisture.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. Prepare solutions fresh before use.
Protocol (For Reference Only)
Important: STZ is light-sensitive and moisture-sensitive. Prepare solutions fresh in sterile, cold citrate buffer (pH 4.5) immediately before use. Handle with appropriate safety precautions as STZ is a potential carcinogen.
1. Solution Preparation: Dissolve STZ in sterile, cold 0.1 M citrate buffer (pH 4.5) to the desired concentration. Filter sterilize through a 0.22 µm membrane. Use within 15-30 minutes of preparation, as STZ degrades rapidly in solution.
2. Type 1 Diabetes Model (Mouse): Administer STZ intraperitoneally at 40-50 mg/kg/day for 5 consecutive days, or at a single high dose of 150-200 mg/kg. Monitor blood glucose levels 48-72 hours after the final injection and confirm diabetes induction (blood glucose >250 mg/dL).
3. Type 2 Diabetes Model (Mouse): Combine a high-fat diet with low-dose STZ (e.g., 30-50 mg/kg/day for 3-5 days, or a single dose of 100-120 mg/kg) to induce insulin resistance and ¦Â-cell dysfunction.
4. Pancreatic Islet Studies: Use lower STZ doses (e.g., 25-40 mg/kg) to induce partial ¦Â-cell damage for studying islet regeneration and ¦Â-cell function.
5. Cancer Research: Use STZ at concentrations of 0.1-5 mM in cell culture for DNA damage and alkylation studies.
Precautions
1. STZ is a potential carcinogen and teratogen; handle with appropriate protective equipment, including gloves, laboratory coat, and eye protection.
2. Prepare solutions fresh before use; STZ degrades rapidly in aqueous solution, particularly at neutral or alkaline pH.
3. Use citrate buffer (pH 4.5) for dissolution to maintain stability.
4. Monitor animals closely after STZ administration for signs of hypoglycemia and dehydration.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the mechanism of STZ-induced diabetes?
A1: STZ is selectively taken up by pancreatic ¦Â-cells via GLUT2 and induces DNA alkylation, nitric oxide production, and oxidative stress, leading to ¦Â-cell necrosis and insulin deficiency.
Q2: How should I prepare STZ solution?
A2: Dissolve STZ in sterile, cold 0.1 M citrate buffer (pH 4.5) immediately before use. Filter sterilize and use within 15-30 minutes, as STZ degrades rapidly in solution.
Q3: What doses are used to induce diabetes in mice?
A3: For type 1 diabetes, 40-50 mg/kg/day for 5 consecutive days or a single dose of 150-200 mg/kg is commonly used. For type 2 diabetes models, low-dose STZ (30-50 mg/kg) combined with a high-fat diet is used.
Q4: How should I store STZ?
A4: Store at -20¡ãC under inert atmosphere, protected from light and moisture. Prepare solutions fresh before use.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of animal experiments, optimization of doses and administration schedules is recommended for specific animal strains and experimental objectives.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product. STZ is a potential carcinogen; review the Safety Data Sheet before handling.
Ordering Information
Catalog Number: IC-03205403
Product Name: Streptozotocin
Size: 50 mg / 100 mg / 250 mg / 500 mg / 1 g
Suggested Retail Price:
50 mg: CNY ¥450.00 / USD $45.00 / EUR €54.00 / JPY ¥8100.00
100 mg: CNY ¥595.00 / USD $59.50 / EUR €71.40 / JPY ¥10710.00
250 mg: CNY ¥1250.00 / USD $125.00 / EUR €150.00 / JPY ¥22500.00
500 mg: CNY ¥1950.00 / USD $195.00 / EUR €234.00 / JPY ¥35100.00
1 g: CNY ¥3450.00 / USD $345.00 / EUR €414.00 / JPY ¥62100.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
